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Êý×Öµ°°×оƬ-- ProteomeChip

By providing differences of 0.05 pH units between adjacent gel plugs, the digital
ProteomeChip enables separation of proteins and peptides often found in the
same fraction by other technologies. High-resolution fractionation facilitates study
of charge isoforms, allows separation of high and low abundance species, and
analysis of high molecular weight proteins.

  • Separate proteins or peptides in 30–60 minutes
  • Compatible with both simple and complex samples
  • Identify the pI of specific proteins and peptides
  • Eliminate variability associated with gel band cutting
  • Achieve 105 depth of coverage
dPCÊý×Öµ°°×ÖÊоƬ·ÖÁóÆ÷
                            Êý×Óµ°°×оƬ

 

dPCÊý×Öµ°°×ÖÊоƬ·ÖÁóÆ÷
Separation of alpha 2-macroglobulin and PKDP1 by pI in plasma, processed using the dPC platform.

Mass Spec Results Analysis Tool (MSRAT®)

BioAnalysis Software

The large data sets generated in mass spec-based proteomics studies can often obscure meaningful experimental findings. MSRAT software streamlines analysis of these data sets and finds biologically significant features minutes after protein database searching. For proteins and peptides separated using dPC, analytical information such as pI isoforms, post-translational modifications and expression levels can also be assessed.

  • Merge multiple MS data sources into a single, organized data set
  • Multiple visualization tools highlight data trends immediately
  • Virtual 3D and differential heat maps simplify comparative analysis
  • Assess peptide coverage, collapse redundancies and group similar proteins
  • Interactive data sorting and graphing features provide real-time results
  • Data are easily exported to Excel or XML
3D Differential Map
dPCÊý×Öµ°°×ÖÊоƬ·ÖÁóÆ÷
  • See fraction by fraction results for individual proteins.
  • Instant access to primary sequence information in NCBI and SwissProt databases.
  • Differential proteins are revealed instantly from protein ID, MW and pI data.
  • Click on a bubble to reveal detailed protein information.
  • Red bubbles indicate underexpressed proteins, overexpressed proteins are blue.
Peptide Analysis
dPCÊý×Öµ°°×ÖÊоƬ·ÖÁóÆ÷
Determine peptide coverage of identified proteins

 
Potential Biomarker Identification
dPCÊý×Öµ°°×ÖÊоƬ·ÖÁóÆ÷
Bar, line and pie charts provide greater flexibility for discovery
 


dPc Fractionator Consumables / Reagents

dPC Analysis Kits


Part #Description
80-0001pH 4.2–6.2 Protein Analysis Kit
80-0002pH 6.0–8.0 Protein Analysis Kit
80-0003pH 7.2–9.2 Protein Analysis Kit
80-0034pH 4.2–6.2 Peptide Analysis Kit
80-0035pH 6.0–8.0 Peptide Analysis Kit
80-0045pH 3.5–4.5 Peptide Analysis Kit

dPC Fractionator Accessories

System Accessories


Part #Description
70-0002dPC Plug Harvester
Removes plugs from dPC chips. Allows plugs to be selected individually or grouped together into the same tube or microplate for custom fraction sizes. Includes dPC holder and harvesting needle.

iWB Blotting System


Part #Description
70-0003iWB Semi-Dry Blotting System
Includes semi-dry transfer device, dPC chip frame, dPC straightener tool and blot roller. Power supply not included.
80-0019iWB Blotting Kit
Includes 6 cathode buffer packs, 6 individual anode pads with PVDF membrane and transfer gel.

Spare Parts


Part #Description
60-0011dPC Chamber Electrode Assembly – Protein
Includes set of 6 running chambers and loading tray.
60-0012dPC Chamber Electrode Assembly – Peptide
Includes set of 6 running chambers and loading tray.

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