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• For investigating the tone of small blood vessels under the effect of vasoactive substances
• Compact arrangement
• Organ is in warm and moist environment during perfusion
• Electrical field stimulation capabilities
The rat hind leg and the rabbit ear are conventional preparations for investigating the tone of blood vessels under the effect of vasoactive substances in the peripheral vascular bed, either at constant perfusion pressure or at constant flow. Both set-ups suffer from the disadvantage that the measurement is affected by early edema formation. In the rat, the mesenteric circulation receives approximately 20% of the cardiac output; its regulation may therefore contribute significantly to the systemic blood pressure and circulating blood volume. The presence of arterioles, i.e. the most important vessels in the regulation of vascular resistance, renders the mesenteric bed an important preparation for the study of vascular physiology, pathophysiology and pharmacology.
The organ bath for the rat mesenteric bed consists of a moist chamber with cover, both water jacketed. The mesenteric tissue is placed into the moist chamber on a stainless steel mesh which also acts as anode during
electrical stimulation. The superior mesenteric artery is cannulated (steel cannula) and perfused at constant flow (e.g. 5 ml/min) through a roller pump via a heat exchanger included in the moist chamber. The test drug is introduced through a separate roller pump or a syringe pump directly before the vascular bed preparation. The perfusion pressure is measured in a branch before the stainless steel cannula. For electrical stimulation, the Mesenteric Bed is placed on a stainless steel grid and a metal clip is attached to the aorta section near the steel cannula.
References:
1) Cusma-Pelogia, N. et al.: Endothelium Inactivation in In Vitro Perfused Vascular Beds - Comparison of Methods. J. Pharmacol and Toxicol. Methods 29, 157-163 (1993)
2)